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| In Vivo Measurement of Chlorophyll
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Chlorophyll, in various forms, is bound within the living cells of algae, phytoplankton, and other plant matter found in environmental water. Chlorophyll is a key biochemical component in the molecular apparatus that is responsible for photosynthesis, the critical process in which the energy from sunlight is used to produce life-sustaining oxygen. In general, the amount of chlorophyll in a collected water sample is used as a measure of the concentration of suspended phytoplankton, the magnitude of which can significantly affect the overall quality of the water. The use of the measurement of phytoplankton as an indicator of water quality is described in Section 10200 A. of Standard Methods for the Examination of Water and Wastewater (Standard Methods). The classical method of determining the quantity of chlorophyll at a particular site is to collect a fairly large water sample and analyze it in the laboratory. The procedure involves filtration of the sample to concentrate the chlorophyll containing organisms, mechanical rupturing of the collected cells, and extraction of the chlorophyll from the disrupted cells into the organic solvent, acetone. |
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As terrestrial sources of oil diminish, the oil industry has moved its exploration further and further offshore in their quest for new fields. Whilst much has been learned over decades about sub-sea production from near-shore sites such as Gulf of Mexico, North Sea, Black Sea and Australia, new problems are being encountered as exploration moves further offshore. If the forecast holds true that known oil reserves will run out within 40 years, then exploitation of deep-sea methane hydrates may take the industry into waters over 4 kilometres deep. All this has resulted in a greater demand for oceanographers to help in overcoming a range of problems.
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| The Development of Seawater Standards for Dissolved Nutrients
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Mass balance equations have shown that the input of the major biologically active nutrients (nitrogen, phosphorous and silicon) to the ocean plays an important role in regulating global oceanic production. Once in the marine system, nutrients are made available to biological organisms for primary production either through nutrient-rich water being drawn up from below, or by local regeneration resulting from cell breakdown. In surface waters of the open ocean, uptake of nutrients by organisms usually results in one or more of those nutrients becoming limiting to their growth (i.e. when the nutrient is used up, production ceases). However, coastal and estuarine waters are increasingly subject to anthropogenic input stress, whereby frequent input of nutrients as run-off from agricultural land results in artificially prolonged algal growth. In either case, the need for high-quality measurement of nutrients is driven by economic factors associated with climate change, depleted fisheries, eutrophication and aquatic ecosystems being out of balance.
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